Suramin decreased 1,25(OH)2D3-upregulated M2 markers (Arg-1, CD163) while increased M1 marker iNOS level when co-cultured with HCC cells (PLC/PRF-5 or Huh7) (p < 0.001) (Figures 4C,D). This evidence concerns the gene ARG1 and hepatocellular carcinoma.