Through the inhibition of the PI3K/Akt pathway, the activity of eNOS decreases by the BTKi, creating an imbalance between ET-1 and the production of NO, leading to endothelial dysfunction and the promotion of vasoconstriction (Byrd et al., 2021; Slowinski et al., 2007). The gene discussed is NOS3; the disease is endothelial dysfunction.