This study aimed to further clarify the effects of this virus on promoting the apoptosis of MCF-7 cells 48 hours after Ad-VT (MOI of 100) or Ad-VP3 (MOI of 100) infection, and transmission electron microscopy was used to observe typical cytoplasmic organelles and nuclear fragments of the apoptotic body, confirming that the recombinant adenoviruses can cause MCF-7 cell apoptosis. Here, UBN1 is linked to infection.