Despite asynchronous cells comprising ∼65% G0/G1 cells versus 35% SG2M cells, we found that in asynchronous ASCL1 ChIP-seq data, peaks enriched for ASCL1 binding in G1 showed generally lower levels of ASCL1 binding compared to those enriched in SG2M (Fig. 4C), which was replicated in two other neuroblastoma cell lines (Fig. S4B). This evidence concerns the gene ASCL1 and neuroblastoma.