ASCL1 and neuroblastoma: We performed ASCL1 ChIP-seq in another MYCN-amplified neuroblastoma cell line (IMR-32) and a non MYCN-amplified cell line (SH-SY5Y), showing that ASCL1 binding at loci associated with both proliferative and neuronal genes is a general feature of ASCL1 functionality in neuroblastoma (Fig. S1C).