While there were no obvious morphological changes in these cells (Fig. S1D,E), knockout of ASCL1 significantly reduced the growth rate when compared to wild-type cells (Fig. 2B), indicating that ASCL1 indeed plays a role in promoting cell cycle progression, as has been seen in other neuroblastoma cell lines (Parkinson et al., 2022). Here, ASCL1 is linked to neuroblastoma.