GAD1 and Alzheimer disease: 2014); however, the expression of GABAergic signaling in this brain area during REM‐SD in the 3xTg‐AD mice has not been investigated yet. Therefore, we conducted immunohistochemistry and found that the interaction between the genotypes and SD induction was statistically significant for the expression of the GABA (F(3, 35) = 5.771, p < 0.022), GAD67 (F(3, 36) = 10.024, p < 0.003), and GABABR1 receptor (F(3, 36) = 20.271, p < 0.001) in the PFC but not the GABAAα1 receptor (F(3, 36) = 1.369, p = 0.251).