Therefore, in the present study, we aim to investigate the relationship between REM‐SD, GABAergic signaling‐related molecular expressions (GABAAα1, GABABR1, glutamate decarboxylase 67 (GAD67), and GABA), and WM performance in the PFC and hippocampal regions of six‐month‐old WT and 3xTg‐AD mice. Here, GABBR1 is linked to Alzheimer disease.