The results demonstrated that the promoter methylation levels of BRAF, EIF1 AX, FOXE1, KRAS, PDGFRA, PIK3 CA, PTEN, and TERT genes in thyroid cancer cell line samples (n = 12) were significantly (p-value < 0.05) higher compared to control cell line samples (n = 06), as illustrated by the beta values representing the degree of methylation (Fig. 2). The gene discussed is PTEN; the disease is thyroid cancer.