Protein level of PFN1 at 24 h post-AOM decreased by 36% (from 103.8% ± 16.95 in control to 66.75% ±8.64; n = 8 and 7 in control and AOM, respectively) while it was not changed at the earlier time points (Fig. 3C). The gene discussed is PFN1; the disease is infectious otitis media.