We measured the levels of AQP4 isoforms mRNA (Fig. 3A) and protein level of AQP4, ezrin, and PFN1 and analysed fluorescence intensity of phosphorylated ezrin; (n = 11 and 3 in control and AOM-injected, respectively; n = 11 and n = 4 in control and AOM-injected, respectively; n = 7 and 3 in control and AOM-injected, respectively; n = 7 and n = 5 in control and AOM-injected, respectively). Here, EZR is linked to infectious otitis media.