The present study was devoted to the dual mechanism of circSLC22A3 in ESCC squamous carcinoma and further examined the expression levels of miR-19b-p, TRAK2 and ACSBG1 in the tissues of the metastatic model mice, and the RT-qPCR results showed that the overexpression of circSLC22A3 resulted in the up-regulation of the expression levels of circSLC22A3 and TRAK2. Here, TRAK2 is linked to squamous cell carcinoma.