After knockdown and overexpression of ACSBG1 in ESCC cells, respectively, the results of Transwell and wound healing assays showed that the migration and invasion abilities of ESCC cells were decreased after knockdown of ACSBG1 and enhanced after overexpression of ACSBG1 (Fig. S7A-D). Here, ACSBG1 is linked to esophageal squamous cell carcinoma.