As LY9 operates in a CD4+ T-cell-intrinsic fashion to promote IFN-γ production, we investigated the functional consequences of LY9 costimulation in cis by RNASeq on LY9 KO HuT78 T-lymphoma cells transduced with EV or WT LY9 and stimulated with microbeads conjugated with anti-LY9 mAb or its isotype control (mouse IgG1), together with an anti-CD3 mAb. This evidence concerns the gene CD4 and lymphoma.