In addition, the enrichment study indicated that S. mannii aqueous roots extract exerts its anticancer activity against cervical cancer by targeting several signalling pathways associated with cell survival, growth, proliferation, regulation of cell cycle, apoptosis, and migration, including HIF-1 (hsa04066), mTOR (hsa04150), PI3K-Akt (hsa04151), Rap1 (hsa04015), MAPK (hsa04010), ErbB (hsa04012), Ras (hsa04014), cAMP (hsa04024), AMPK (hsa04152), FoxO (hsa04068), chemokine (hsa04062), JAK-STAT (hsa04630), NF-kappa B (hsa04064) and P53 (hsa04115) signalling pathways. This evidence concerns the gene HIF1A and cervical cancer.