In addition, the enrichment study indicated that S. mannii aqueous roots extract exerts its anticancer activity against cervical cancer by targeting several signalling pathways associated with cell survival, growth, proliferation, regulation of cell cycle, apoptosis, and migration, including HIF-1 (hsa04066), mTOR (hsa04150), PI3K-Akt (hsa04151), Rap1 (hsa04015), MAPK (hsa04010), ErbB (hsa04012), Ras (hsa04014), cAMP (hsa04024), AMPK (hsa04152), FoxO (hsa04068), chemokine (hsa04062), JAK-STAT (hsa04630), NF-kappa B (hsa04064) and P53 (hsa04115) signalling pathways. The gene discussed is TP53; the disease is cervical cancer.