To validate the efficacy of S. mannii aqueous roots extract against cervical cancer, qRT-PCR was used to assess the expression of some of the core targets identified in the network pharmacology analysis (NFKB1, PIK3CA, HIF1A, STAT3, HSP90AA1, HSP90AB1, PPARG, and ESR1), following treatment of HeLa cells with S. mannii aqueous roots extract in a time- and dose-dependent manner (S3 Table in S1 File). The gene discussed is ESR1; the disease is cervical cancer.