However, pre-treatment with 50 μM Hesperidin and 0.25 μM DRB reduced apoptosis by lowering the Bax/Bcl-2 ratio compared to the Aβ1–42 group (p < 0.01 and p < 0.05, respectively) in the in vitro AD model (Fig. 6C). The gene discussed is BCL2; the disease is Alzheimer disease.