In agreement with the results shown in Fig. 1e, the presence of anti-ACE2 antibodies in culture media before and during infection drastically inhibited infectivity (Fig. 2g) as evidenced by reduction in the eGFP expression (in all cultures) and Spike expression (in four out of six cultures) at 48 h p.i. Furthermore, susceptibility to infection was also reduced in cultures treated with the inhibitor of TMPRSS2, camostat mesylate as determined by eGFP and Spike transcript levels (Fig. 2h), although the degree of inhibition varied between cultures derived from different tissue specimens. This evidence concerns the gene TMPRSS2 and infection.