An increase in ECAR was noted both in control and GLUT1-transduced TC-1 cells, particularly in those overexpressing GLUT1, demonstrating a negative correlation between high GLUT1 expression and tumor-infiltrating lymphocyte (TIL) infiltration in HNSCC, which delayed tumor regression (Figure 2) (22). Here, SLC2A1 is linked to head and neck squamous cell carcinoma.