A total of 5 × 106 FZD2 knockout (KO) AGS and HGC-27 gastric cancer cells were resuspended in sterile PBS and mixed with Ceturegel® matrix gel before being subcutaneously injected subcutaneously into the flank area of 6–7-week-old male BALB/c-nude mice (n = 3 per group). The gene discussed is FZD2; the disease is gastric cancer.