To investigate the role of TP in modulating the expression of RANKL by osteoblasts in the tumor microenvironment, mouse osteoblast precursor cells MC3T3-E1 were cultured in CM, with or without TP treatment, and determined the protein and transcriptional expression of RANKL in MC3T3-E1 cells. The gene discussed is TNFSF11; the disease is neoplasm.