Serum levels of DGKK and S10A8 in pregnant women with hypothyroidism (Group H) and normal pregnant women (Group C) during pregnancy were determined using ELISA, and as shown in Figure 5, serum DGKK and S10A8 were significantly downregulated in the hypothyroidism group compared with the normal group (p < 0.05). The gene discussed is DGKK; the disease is hypothyroidism.