While our in vitro experiments using C1R-A24 cells pulsed with long peptides and 293-A24 cells overexpressing TMG encoding the peptides demonstrate that APC frameshift neoantigen peptides are intracellularly processed and presented on HLA-A24:02 molecules on the cell surface, we were unable to fully recapitulate endogenous antigen presentation in CRC cells due to the lack of suitable CRC cell lines with the relevant APC mutations and HLA-A*24:02 expression. The gene discussed is HLA-A; the disease is colorectal carcinoma.