Considering that the proliferation and invasion ability of BC cells will be affected after apoptosis, combined with the inhibition of AKT/FOXO3 A signaling pathway and the changes of related biological behaviors, it is suggested that the proliferation and invasion of BC cells may be the result of both direct targeting AKT/FOXO3 A pathway by SHMT1 and indirect inducing apoptosis. This evidence concerns the gene SHMT1 and breast cancer.