CTNNB1 and hepatocellular carcinoma: KEGG and GO pathway enrichment analysis on the reprogrammed hepatocytes demonstrated enrichment of pathways across all zones, including biosynthesis of cofactors (Zone 1), amino acid catabolism (Zone 1), arginine biosynthesis (Zone 1), glutamate metabolism (Zone 3), glycolysis/TCA cycle (Zone 3), along with fatty acid metabolism, a hallmark of CTNNB1-mutated hepatocellular cancers (Supplementary Fig. 9a–d).