KEGG pathway enrichment analysis comparing each cluster to all other clusters revealed that top pathways for Zone 3 CTNNB1 WT & MUT (GS+) hepatocytes were bile acid secretion, drug metabolism – cytochrome P450, and fatty acid metabolism, which are all known hallmarks of CTNNB1-mutated HCC (Supplementary Fig. 8d)35. Here, CTNNB1 is linked to hepatocellular carcinoma.