Moreover, H2S induces sulfhydrylation of Kelch-like ECH-associated protein 1 (Keap1) at Cys151, promotes its association with (NF-E2)-related factor 2 (Nrf2), and increases the expression of Nrf2-associated antioxidant genes in vivo and in vitro, thereby improving liver function and reducing liver fibrosis.250 S-allyl-cysteine (SAC) is an endogenous donor of H2S, which can alleviate carbon tetrachloride (CCL4)-induced liver fibrosis in rats, reduce the mRNA expression of inflammatory and fibrotic cytokines, and increase antioxidant enzyme activity. The gene discussed is NFE2L2; the disease is Hepatic fibrosis.