Furthermore, when expressed in GC and CRC cells, the CAD-D1371E or CAD-D1371Y mutants demonstrated significant protection against cell death and inhibited H2A.X phosphorylation when treated with 5-FU, compared to the wildtype CAD (Fig. 5d–g and Supplementary Fig. 5j, k). This evidence concerns the gene H2AX and colorectal carcinoma.