GSDME and colorectal carcinoma: Indeed, various GC and CRC cell lines, including the HGC27, MKN45, HCT116, and SW480 analyzed in this study, showed positivity for gasdermin-E (GSDME) as determined by immunoblotting (Supplementary Fig. 7g, where the non-small cell lung cancer (NSCLC) cell line NCI-H1299, known to express high levels of GSDME, was used as a positive control).