Previous evidence has shown that the levels of GATA3, IL-18, TLR4, TNFSF12A, and CD68 are negatively associated with the mean esophageal pH;[6] and that the IL-10 and IL-8 are associated with histological alterations in the esophageal lining.[39] The levels of IL-8, serum IL-10, and TNFSF12-a grow by a degree in patients with GERD as disease progresses.[40] Furthermore, clinical evidence suggests that serum interleukin-6, IL-8, TNFSF12-a-levels are highly valuable for assessing the severity of GERD. This evidence concerns the gene TLR4 and gastroesophageal reflux disease.