For the anxiety-like behaviors indicated by the percentage of time spent in the open arms of the elevated plus maze test, ACTH of HPA axis (Figure 11A), LH of HPT axis (Figure 11B) and GnRH of HPO axis (Figure 11C) (GnRH>LH>ACTH) dedicated to anxiety and respond to the treatment of QFY in male mice. The gene discussed is PLOD1; the disease is Anxiety.