Bulk RNA-seq of human and canine insulinoma and bulk ATAC-seq of human insulinoma has already provided valuable insights into the global regulatory, mutational and transcriptomic features of insulinoma [8, 33], but here we apply scRNA-seq analysis to canine insulinoma and identify two distinct subtypes of insulin-expressing cells within canine insulinoma for the first time. The gene discussed is INS; the disease is pancreatic insulinoma.