Using qRT-PCR, we observed a decrease in E-cadherin and an increase in mesenchymal markers such as Slug, N-cadherin, vimentin, ZEB1, and ZEB2 in LNCaP cells treated with periprostatic tumor fat medium, indicating epithelial–mesenchymal transition (Fig. 5E,F). This evidence concerns the gene ZEB2 and neoplasm.