Alongside with NGS, imaging-based approaches, such as fluorescence in situ hybridization (FISH), immunohistochemistry, and electron microscopy are commonly used to assess TRB presence and abundance in cancer specimens (Fu et al, 2022; Galeano Niño et al, 2022; Lu et al, 2024; Nejman et al, 2020). The gene discussed is THRB; the disease is cancer.