To acutely induce the formation of CIs in human cultured cells, we transiently transfected HeLa cells with plasmids expressing wild-type (WT) FUS and its ALS-linked mutant form (FUSP525L), along with WT TDP-43 and two ALS-related mutant derivatives (TDP-43A382T and TDP-43I383V), in parallel with an empty vector (EV) as a control. Here, TARDBP is linked to amyotrophic lateral sclerosis.