Moreover, our in vitro findings showed that VEGF secreted by B16-F10 cells perturbs endothelial cell junctions in a manner that facilitates the passage of tumor cells across endothelial cell monolayers and that VIAN-c4551 prevented melanoma cell-induced vascular leakage like the anti-VEGF antibody fragment ranibizumab, which blocks the interaction of VEGF with its receptor [45]. This evidence concerns the gene VEGFA and neoplasm.