Colorectal adenocarcinoma cell lines were deliberately chosen to encompass a range of CHRDL2 and BMP expression levels, as well as genetic mutations: CACO2 and LS180 (moderate CHRDL2), COLO320 and RKO (very low) (Fig. S1A–C). This evidence concerns the gene CHRDL2 and colorectal adenocarcinoma.