Utilizing severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) spike 1 (S1) protein and ovalbumin (OVA) as model antigens, ADN achieves robust production of anti-S1 subunit IgG antibody and potently suppresses the growth of tumors and LN metastasis in a murine model of OVA-overexpressed melanoma. This evidence concerns the gene PSMD1 and melanoma.