As shown in Figure 6e,f, western blot analysis showed that the phosphorylation of MEK1, Erk1/2, IκBα and NF‐κB and the expression of TNF‐α were significantly downregulated by CCS‐RSF@PRP hydrogel treatment, compared with that of periodontitis group, indicating that inflammatory regulation effect of CCS‐RSF@PRP hydrogel was potentially transmitted through TNF signaling pathway. This evidence concerns the gene MAPK3 and periodontitis.