Western blot analysis showed that QSOX2 overexpression significantly increased the phosphorylation levels of TSC2Ser939, mTORSer2448, 4E‐BP1Thr37/46 and expression level of c‐Myc in ESCC cells, while the levels of p‐TSC2Thr1462 and p‐TSC2Ser1387 remained unchanged (Figure 4E; Figure S5D, Supporting Information). This evidence concerns the gene QSOX2 and esophageal squamous cell carcinoma.