CFTR and cystic fibrosis: To deeply explore the association between this case and CF, this study employed multiple mutation prediction tools such as Mutation Taster and PolyPhen-2, as well as molecular dynamics (MD) simulation techniques, to analyze the impact of the above-mentioned compound missense mutations on the function of the CFTR protein, aiming to provide new insights for the diagnosis, treatment, and genetic counseling of CF caused by the complex and diverse mutations of CFTR.