CD69 and neoplasm: Following co-culture with different tumor targets, we noted that Siglec-7-based CSR could trigger an upregulation of these markers compared to TCR-only control; for example, S7-41BB leads to 43% more expression CD69, 20% more 4-1BB, and 12% more CD25 expression in cocultures with 888/A2 (Figures 2D–F).