Accordingly, in vitro transcribed hsRNAs with a 30 nt 3′ poly(A) tail were transfected into wildtype, ZCCHC14-depleted (ZCCHC14-KO), or TENT4A/B-depleted (TENT4A/B-KO) Huh-7.5 cells [4], and this was followed by infection with the 18f-NLuc reporter virus for 72 h. Here, TENT4A is linked to infection.