Next, we performed a subgroup analysis and interaction test to evaluate the relationship between TMAO, choline, betaine, L-carnitine, and MACE and potentially modifying factors, including age, sex, type of MI, hypertension, diabetes mellitus, previous MI, previous PCI and CABG, previous stroke, smoking and alcohol consumption, LVEF, blood creatinine, LDL-C, peak cardiac troponin I, number of diseased vessels, emergency and elective PCI, and standard medication for prevention. This evidence concerns the gene TNNI3 and myocardial infarction.