INS and fatty liver disease: In this sense, various studies carried out in rodent models have highlighted that exposure to high-calorie diets for specific periods of time (2–22 weeks) produces the highest serum levels of cholesterol, triglycerides, LDL cholesterol, resistance to insulin and liver enzymes (ALT and AST), severe macrovesicular hepatic steatosis and periportal inflammation [15], gradual development of NAFLD (characterized by sustained increases in ALT, HOMA-IR, serum cholesterol, and TNF-α), grade 2–3 hepatic steatosis, lobular inflammation, and the appearance of perisinusoidal fibrosis [16].