(Key findings) Treatment with berberine and carvacrol significantly reduced tissue injury associated with endometritis, decreased mRNA expression of TLR2 and TLR4 (p < 0.01), and inhibited the phosphorylation of NF-κB and MAPK pathway-associated proteins, as well as the mRNA expression and levels of pro-inflammatory cytokines. The gene discussed is TLR4; the disease is endometritis.