Western blot analysis revealed increased TGF-β1 levels and activation of the SMAD2/3 pathway in CCNB1-overexpressing melanoma cells (Figure 8C), whereas CCNB1 knockdown led to reduced TGF-β1 expression and inhibition of SMAD2/3 signaling (Figure 8D). This evidence concerns the gene TGFB1 and melanoma.