To investigate curcumol’s mechanism of action through OTUB1 modulation in colorectal cancer and angiogenesis, we generated OTUB1 overexpression (OTUB1-OE) and control (Control-OE) plasmids, which were transfected into human colorectal cancer cell lines (HT-29 and Caco-2) and human umbilical vein endothelial cells (HUVECs). Here, OTUB1 is linked to colorectal cancer.