To further examine the synergistic effects of 2-DG and diclofenac sodium on the apoptosis and metastasis of breast cancer cells, Western blotting was performed to examine the effects of a combined treatment of the drugs on the expression of HIF-1α and the cleavage of pro-apoptotic proteins, Caspase-3 (Cas-3) and Caspase-9 (Cas-9). This evidence concerns the gene CASP3 and breast cancer.