Unlike what was observed for NEAT1, we did not detect significant changes in ZBTBT11-AS1 on days 4 and 21 p.i. Additionally, while nuclear levels of ZBTB11-AS1 decreased on day 4 p.i., no significant nuclear changes were observed on day 21 p.i. Although our study did not establish a definitive role for ZBTB11-AS1, identifying its presence in both the nucleus and cytoplasm of infected microglia, as well as noting the changes during the viral replication stages, is a step forward in exploring the involvement of lncRNAs in HIV-1 infection in C20 microglial cell lines. The gene discussed is ZBTB11; the disease is HIV-1 infection.