To further investigate the putative relationships between HIV-1 in microglia and lncRNAs, we measured the total, nuclear, and cytoplasmic levels of NEAT1 and ZBTB11-AS1 at 4 and 21 days post-HIV-1 infection in the C20 microglial cell line—a human immortalized microglial cell line derived from fresh CNS tissue used to understand viral infection with HIV-1 and Chikungunya virus (CHIKV) [34,35,36]. This evidence concerns the gene ZBTB11 and HIV-1 infection.