Figure 3 demonstrates a representative illustration of ICC staining targeting 12 proteins in the related BC cell lines. This series of experiments revealed that 3 out of 12 markers (MARCKSL1, SLC9A3R1, and RHOD) had a higher expression level and were specific to cancer cells (Table 1). The gene discussed is MARCKSL1; the disease is intrahepatic cholangiocarcinoma.