As the evaluation of sperm parameters revealed a decreased sperm motility in T1D rats, we decided to further explore this aspect by the analysis of acetylated tubulin (K-TUB), a well-known marker of sperm motility [54], along with Alpha-Tubulin N-Acetyltransferase 1 (ATAT1) and Histone Deacetylase 6 (HDAC), the main TUB acetyltransferase [55] and deacetylase [56], respectively. This evidence concerns the gene NAT1 and type 1 diabetes mellitus.