FLIM reveals metabolic alterations through NAD(P)H autofluorescence patterns, with IDH-mutant oligodendrogliomas demonstrating significantly shorter lifetimes (3.3 ± 0.1 ns) compared to IDH-mutant astrocytomas (4.1 ± 0.1 ns) [40]. This evidence concerns the gene IDH1 and astrocytoma (excluding glioblastoma).