Defensins 5 and 6, I-FABP and L-FABP in children were chosen to assess the translocation of bacterial flora because they provide specific information on intestinal integrity, microbial defense and liver–gut interactions, which are very important in the development and progression of metabolic disorders in children, especially metabolic dysfunction-associated steatotic liver disease (MASLD) [6,7]. Here, DEFA5 is linked to metabolic dysfunction-associated steatotic liver disease.