Next, to achieve tumor cell-specific knockout of Olig1/2 and enable labeling of the knockout cells, we first crossed Olig1/2F/F mice with two distinct reporter systems: ISF/+ mice [28] (membrane-localized tdTomato labels the cell membrane) and H2bF/+ mice [36] (nuclear-localized GFP marks the nucleus). Here, OLIG1 is linked to neoplasm.