Identified 92 genetic variants; 18 were novel. Pathogenic and likely pathogenic variants found primarily in LMF1. Genetic variation in LMF1 significantly associated with sHTG. Highest ever triglyceride levels, significantly higher among participants with a history of pancreatitis (4317 mg/dL) compared to those without pancreatitis (1769 mg/dL). Suggests that genetic variants, particularly in LMF1, play an important role in the pathogenesis of sHTG in Colombian populations. Here, LMF1 is linked to pancreatitis.