However, in PDA cells transfected with a lentivirus to interfere with the expression of the PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1) gene, there were no significant differences in apoptosis rates or malignancy biomarker expression between the isoorientin-treated and untreated groups [117]. The gene discussed is PRKAA1; the disease is Patent ductus arteriosus.