As illustrated in Figure 4M, we concluded that rG4-containing TCNEs were not only involved in pathways that could promote tumor initiation and progression (such as Notch signaling, Hedgehog signaling, KRAS signaling DN, P53 pathway, and Wnt/β-catenin signaling), but also associated with estrogen response in breast cancer. The gene discussed is KRAS; the disease is breast cancer.